Useful for Medical Entrance Exams: AIPMT / AFMC /JIPMER / AIIMS / JIPMER /Manipal UGET / CMC Vellore / AMU/ BHU

Multiple Choice Questions on Biotechnology

1. Genetic engineering is possible, because
a) We can cut DNA at specific sites by endonucleases like DNAase I
b) Restriction endonucleases purified from bacteria can be used in vitro
c) The phenomenon of transduction in bacteria is well understood
d) We can see DNA by electron microscope
2. Restriction endonucleases (Restriction enzymes) are used for
Genetic Engineering
a) Used to invitro DNA synthesis
b) Used in genetic engineering
c) Synthesized by bacteria
d) Present in mammalian cells for degradation of DNA

3. Transgenic animals are those which are
a) Foreign RNA in all its cells
b) Foreign DNA in some of its cells
c) Foreign DNA in all its cells
d) Both a and b

4. The first transgenic plant was
a) Tobacco
b) Pea
c) Flax
d) Cotton

5. Which of the following is related to genetic Engineering?
a) Plastid
b) Plasmid
c) Heterosis
d) Mutation

6. First cloned animal
a) Dolly sheep
b) Dog
c) Mule
d) Cat

7. Which of the following bases in plasmids discovered so far
a) 50 kilo base
b) 500 kilo base
c) 5 kilo base
d) 500 kilo base


8. Plasmid has been used as vector because
plasmid
a) It has antibiotic resistance gene
b) It is circular DNA which have capacity to join to eukaryotic DNA
c) Both ends show replication
d) It can move between prokaryotic and eukaryotic cells

9. The bacteria generally used for genetic engineering is
a) Bacillus
b) Pseudomonas
c) Clostridium
d) Agrobacterium

10. Which of the following cut the DNA from specific places
a) E.coli restriction endonuclease II
b) Ligase
c) Exonuclease
d) Alkaline phosphatase

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Answers
1. b) Restriction endonucleases purified from bacteria can be used in vitro
2. b) Used in genetic engineering
3. c) Foreign DNA in all its cells
4. a) Tobacco
5. b) Plasmid
6. a) Dolly sheep
7. b) 500 kilo base
8. b) It is circular DNA which have capacity to join to eukaryotic DNA
9. d) Agrobacterium
10.a) E.coli restriction endonuclease II

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