NEET Biology Practice Test on Biotechnology Principles and Processes

Practice test on ‘Biotechnology Principles and Processes’.  Choose the best answer from the four options given. When you've finished answering as many of the questions as you can, scroll down to the bottom of the page and check your answers by clicking ' Score'. Percentage score will be displayed along with right answers.

1.Which of the following is not a cloning vector?
YAC
BAC
Probe
pBR322

2.In gene gun method used to introduce alien DNA into host cells, microparticles of ----metal are used
Zinc
Silver
Copper
Tungsten or gold

3.Upon exposure to UV radiation, DNA stained with Ethidium bromide will show----
Bright blue colour
Bright yellow colour
Bright orange colour
Bright red colour

4.Given below are two statements:

Statement I:Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

Statement II:Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below:

Statement I is correct but Statement II is incorrect
Statement I is incorrect but Statement II is correct
Both Statement I and Statement II are correct
Both Statement I and Statement II are incorrect

5.Which of the following is not a desirable feature of a cloning vector?
Presence of single restriction enzyme site
Presence of two or more recognition sites
Presence of origin of replication
Presence of a marker gene

6. In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

5'CTCAGT 3'; 3'GAGTCA 5'
5'GTATTC 3'; 3'CATAAG 5'
5'GATACT 3'; 3'CTATGA 5'
5'GAATTC 3'; 3'CTTAAG 5'

7.Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A) :Polymerase chain reaction is used in DNA amplification.

Reason (R) :The ampicillin resistant gene is used as a selectable marker to check transformation

In the light of the above statements, choose the correct Ans: from the options given below :

(A) is correct but (R) is not correct
(A) is not correct but (R) is correct
Both (A) and (R) are correct and (R) is the correct explanation of (A)
Both (A) and (R) are correct but (R) is not the correct explanation of (A)

8. Which one of the following statements is not true regarding gel electrophoresis technique
The process of extraction od separated DNA from gel is called elution.
The separated DNA fragments are stained by using ethidium bromide
The separated DNA fragments are stained by using ethidium bromide
bright orange coloured bands of DNA can be observed in the gel when exposed to uv light

9. DNA precipitation out of a mixture of biomolecules can be achieved by treatmnet with-----
methanol at room temperature
chilled chloroform
isopropanol
chilled ethanol

10.Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
Retrovirus
Ti plasmid
λ phage
pBR322
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