NEET Biology Biotechnology and its Applications

Practice test on ‘Biotechnology and its applications’.  Choose the best answer from the four options given. When you've finished answering as many of the questions as you can, scroll down to the bottom of the page and check your answers by clicking ' Score'. Percentage score will be displayed along with right answers.

1.Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early development
Recombinant DNA technology
ELISA
Serum and Urine analysis
PCR technique

2.When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as ---
biopiracy
gene therapy
molecular diagnosis
safety testing

3.With regard to insulin choose the correct options.

I. C-peptide is not present in mature insulin

II. The insulin produced by rDNA technology has C-peptide

III. The pro-insulin has C-peptide

IV. A peptide and B-peptide of insulin are interconnected by disulphide bridges.

Choose the correct answer from the options given below

II and IV
II and III
I, II and IV
I and IV

4.In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because :
Lymphocyctes from patient's blood are grown in culture, outside the body.
Genetically engineered lymphocytes are not immortal cells.
Retroviral vector is introduced into these lymphocytes.
Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages

5.Statements related to human Insulin are given below. Which statement(s) is/are correct about genetically engineered Insulin?

(a) Pro-hormone insulin contain extra stretch of C-peptide

(b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.

(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.

(d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.

(e) Some patients develop allergic reactions to the foreign insulin. Choose the most appropriate answer from the options given below:
(c) and (d) only
(c), (d) and (e) only
(a), (b) and (d) only
(b) only

6. Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell

B. Cutting of DNA at specific location by restriction enzyme

C. Isolation of desired DNA fragment

D. Amplification of gene of interest using PCR Choose the correct answer from the options given below :

C, A, B, D
C, B, D, A
B, D, A, C
B, C, D, A

7.Match the following

A-IV, B-I, C-II, D-III
A-III, B-II, C-I, D-IV
A-II, B-III, C-IV, D-I
A-I, B-II, C-III, D-IV

8. What triggers activation of protoxin to active toxin of bacillus Thuringiensis in boll worm?
acidic pH of stomach
body temperature
alkaline PH of gut
moist surface of midgut

9. In RNAi, the genes are silenced using
ds-RNA
ss-DNA
ds-DNA
ss-RNA

10.Golden rice is genetically modified crop plant where the incorporated gene is meant for biosynthesis of
omega 3
vitamin A
Vitamin C
Vitamin B
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